Vimentin Antibody

SKU: orb44572

Description

Mouse monoclonal antibody to Vimentin

Research Area

Epigenetics, Infectious Diseases, Neuroscience

Images & Validation

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Item 1 of 4

Tested Applications	ICC, IHC-P, IP, WB
Reactivity	Gallus, Human, Mouse, Porcine, Rat
Application Notes	Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line. Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml, positive tissue: skin fibroblast.Western blotting: Recommended dilution: 1-2 μg/ml.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Mouse IgM
Clone No.	VI-10
Target	Vimentin
Purification	Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Concentration	1 mg/ml
Disclaimer	For research use only

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Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody VI-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of vimentin is 57 kDa. Lr = lysate (reducing conditions); Lnr = lysate (non-reducing conditions); IPr = immunoprecipitate (reducing conditions); IPnr = immunoprecipitate (non-reducing conditions).

Immunocytochemistry staining of chicken postnatal erythrocytes. Tubulin (red) was stained with anti-alpha-tubulin (TU-01; cat. no. orb44529), vimentin (green) with anti-Vimentin (VI-10). Nuclei are stained with DAPI (blue).

Immunohistochemistry staining of human skin (paraffin sections) using anti-vimentin (VI-10).

Western blotting analysis of human vimentin using mouse monoclonal antibody VI-10 on lysates of MOLT-4 cell line (low expression), U87 cell line (positive) and Raji cell line (negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-vimentin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for vimentin at approximately 55 kDa.

Quick Database Links

Gene Symbol

Vimentin

UniProt

P08670

NCBI Gene ID

7431

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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ICC

Immunocytochemistry

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Immunoprecipitation

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