AOC3/VAP1 Antibody

SKU: orb1248538

Description

AOC3/VAP1 Antibody

Research Area

Cancer Biology

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Application Notes	Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 90kDa band observed in lysates of cell line A549 (calculated MW of 84.6kDa according to NP_003725.1). Recommended concentration: 0.1-0.3µg/mL. An additional band of unknown identity was also consistently observed at 38kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Goat
Clonality	Polyclonal
Immunogen	The immunogen for this antibody is: ESQAPKTIRDAFC
Target	AOC3 / VAP1
Molecular Weight	Approx 90kDa
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	500 ug/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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AOC3, amine oxidase, copper containing 3 (vascular adhesion protein 1), HPAO, SSAO, VAP-1, VAP1, amine oxidase, copper containing 3, copper amine oxidase, semicarbazide-sensitive amine oxidase, vascular adhesion protein 1

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orb1248538 (0.1 ug/ml) staining of A549 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

AOC3 / VAP1

UniProt

Q16853

RefSeq (Protein):NP_003725.1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_003725.1

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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