APEH Rabbit Polyclonal Antibody

SKU: orb580319

Description

Rabbit polyclonal antibody to APEH

Research Area

Epigenetics & Chromatin, Molecular Biology, Signal Transduction

Images & Validation

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Item 1 of 6

Tested Applications	IHC-P, WB
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Equine, Guinea pig, Rabbit, Rat, Zebrafish

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the N terminal region of human APEH
Target	APEH
Molecular Weight	81kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

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APH, OPH, AARE, ACPH, D3S48E, D3F15S2, DNF15S2

Quality Guarantee

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APEH was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with orb580319 with 1:200 dilution. Western blot was performed using orb580319 at 1/1000 dilution, Lane 1: Control IP in HEK293 Whole Cell Lysate, Lane 2: APEH IP with orb580319 in HEK293 Whole Cell Lysate, Lane 3: Input of HEK293 Whole Cell Lysate.

Sample Tissue: Mouse Brain, Antibody dilution: 1 ug/ml.

Rabbit Anti-APEH Antibody, Catalog Number: orb580319, Formalin Fixed Paraffin Embedded Tissue: Human Adult liver, Observed Staining: Cytoplasmic, Primary Antibody Concentration: 1:600, Secondary Antibody: Donkey anti-Rabbit-Cy2/3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec, Protocol located in Reviews and Data.

WB Suggested Anti-APEH Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: 721_B cell lysate. APEH is supported by BioGPS gene expression data to be expressed in 721_B.

WB Suggested Anti-APEH antibody Titration: 1 ug/ml, Sample Type: Human liver.

Quick Database Links

Gene Symbol

APEH

UniProt

P13798

RefSeq (Protein):NP_001631

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001631

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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