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Description
Research Area
Metabolism Research, Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IF, IHC-P, WB |
|---|---|
| Dilution Range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This APRT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-60 amino acids from the N-terminal region of human APRT. Antigen Region: 27-60 aa. |
| Target | APRT (HGNC:626) |
| Molecular Weight | 19608 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Adenine phosphoribosyltransferase, APRT, APRT
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−- Item 1 of 4
APRT Antibody (N-term) [orb1931357]
FC, IF, IHC-P, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μl, 50 μl
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Flow cytometric analysis of HepG2 cells using APRT Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human hepatocarcinoma with APRT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
All lanes: Anti-APRT Antibody (N-term) at 1:1000 dilution. Lane 1: A431 whole cell lysates. Lane 2: A2058 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 20 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Fluorescent image of A549 cell stained with APRT Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with APRT primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).APRT immunoreactivity is localized to Cytoplasm and Nucleus significantly.
Quick Database Links
Gene Symbol
APRT (HGNC:626)
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
APRT Antibody (N-term) (orb1788008)
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