ASS Antibody (Center)

SKU: orb1929777

Description

Research Area

Metabolism Research, Signal Transduction

Images & Validation

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Item 1 of 7

Tested Applications	FC, IF, IHC-P, WB
Dilution Range	IHC-P-Leica - 1:500, WB - 1:1000-1:2000
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	46530 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Staining ASS in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling ASS at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

Western blot analysis of ASS Antibody (Center) in Hela cell line lysates (35 ug/lane). ASS (arrow) was detected using the purified Pab.

All lanes: Anti-ASS Antibody (Center) at 1:2000 dilution. Lane 1: MOLT-4 whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: NIH/3T3 whole cell lysate. Lane 4: PC-12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-ASS Antibody (Center) at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: MOLT-4 whole cell lysate. Lane 4: NIH/3T3 whole cell lysate. Lane 5: PC-12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-ASS Antibody (Center) at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: MOLT-4 whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: PC-12 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lane 6: Mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 47 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P00966

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

Flow Cytometry

Immunofluorescence