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ATF6 Antibody

SKU: orb1294330

Description

Rabbit polyclonal antibody to ATF6

Research Area

Cell Biology

Images & Validation

Tested ApplicationsIF, IHC, WB
Dilution rangeWestern Blot 1:500–1:1000 Immunofluorescence 1:200–1:300 Immunohistochemistry (Paraffin) 1:200-1:400
ReactivityHuman, Mouse, Rat

Key Properties

HostRabbit
ClonalityPolyclonal
IsotypeRabbit-IgG
ImmunogenSynthetic peptide / encompassing a sequence within the center region
TargetATF6
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
DisclaimerFor research use only

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Quality Guarantee

Quality Guarantee

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ATF6 Antibody

All lanes: Anti-ATF6 antibody - at 1/1000 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294330 overnight at 4°C.

ATF6 Antibody

All lanes: Anti-ATF6 antibody - at 1/1000 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294330 overnight at 4°C.

ATF6 Antibody

All lanes: Anti-ATF6 antibody - at 1/1000 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294330 overnight at 4°C.

ATF6 Antibody

All lanes: Anti-ATF6 antibody - at 1/1000 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294330 overnight at 4°C.

ATF6 Antibody

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294330 anti-ATF6 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

ATF6 Antibody

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling ATF6 with orb1294330 at 1/200.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol

ATF6 Antibody (orb1294330)

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Available Sizes

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25 μl
¥ 3,380.00
100 μl
¥ 5,070.00
DispatchUsually dispatched within 5-10 working days
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