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ATF6 Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution range | Western Blot 1:500–1:1000 Immunofluorescence 1:200–1:300 Immunohistochemistry (Paraffin) 1:200-1:400 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / encompassing a sequence within the center region |
| Target | ATF6 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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All lanes: Anti-ATF6 antibody - at 1/1000 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294330 overnight at 4°C.

All lanes: Anti-ATF6 antibody - at 1/1000 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294330 overnight at 4°C.

All lanes: Anti-ATF6 antibody - at 1/1000 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294330 overnight at 4°C.

All lanes: Anti-ATF6 antibody - at 1/1000 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294330 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294330 anti-ATF6 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling ATF6 with orb1294330 at 1/200.
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ATF6 Antibody (orb1294330)
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