ATG4C Antibody (Center)

SKU: orb1933468

This product can be custom made

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology

Images & Validation

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Item 1 of 3

Tested Applications	IHC-P, WB
Dilution Range	WB - 1:1000, IHC-P - 1:50-100
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	BTD9619
Immunogen	This ATG4C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 167-196 amino acids from the Central region of human ATG4C. Antigen Region: 167-196 aa.
Target	ATG4C {ECO:0000303\|PubMed:21177865, ECO:0000312\|HGNC:HGNC:16040}
Molecular Weight	52497 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Cysteine protease ATG4C, 3422-, AUT-like 3 cysteine endopeptidase, Autophagin-3, Autophagy-related cysteine endopeptidase 3, Autophagy-related protein 4 homolog C, ATG4C, APG4C, AUTL1, AUTL3

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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

The anti-APG4C Pab is used in Western blot to detect APG4C in Hela tissue lysate

Western blot analysis of anti-hAPG4C-I182 Pab in 293 cell line lysates transiently transfected with the ATG4C gene (2 ug/lane). hAPG4C-I182 (arrow) was detected using the purified Pab.

Quick Database Links

Gene Symbol

ATG4C {ECO:0000303|PubMed:21177865, ECO:0000312|HGNC:HGNC:16040}

UniProt

Q96DT6

RefSeq (Protein):NP_835739.1, NP_116241.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_835739.1, NP_116241.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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