ATG5 Antibody (C-term)

SKU: orb1933444

Description

Images & Validation

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Item 1 of 5

Tested Applications	IF, IHC-P, WB
Dilution range	WB - 1:1000, IHC-P - 1:100-500
Reactivity	Human, Mouse

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	32447 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Disclaimer	For research use only

Alternative Names

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APG5L, ASP

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Fluorescent image of U251 cells stained with ATG5 Antibody (C-term). Diluted at 1:25 dilution. U251 cells were treated with Chloroquine (50 μM, 16 h), An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Cos7, HEK293, MEF, and Hela cells left to right, respectively.

Western blot analysis of APG5L Pab in Y79 cell line, mouse liver tissue, and Hela cell line lysates.

Western blot analysis of APG5L (arrow) using rabbit polyclonal APG5L Antibody (P224). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5L gene.

Quick Database Links

UniProt

Q9H1Y0

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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