ATP5F1A Mouse Monoclonal Antibody

SKU: orb394740

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Description

Mouse monoclonal antibody to ATP5A1.

Research Area

Epigenetics

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, FC, IF, IHC, WB
Dilution Range	WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Immunogen	ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
Target	ATP5F1A
Molecular Weight	50-55 kDa
Purity	≥95% as determined by SDS-PAGE
Purification	Protein A+G purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	liquid
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ATP5A, ATP5AL2, ATPM

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Immunofluorescent analysis of Hela cells using orb394740 (ATP5A1 antibody) at dilution of 1:25.

IP Result of anti-ATP5A1 (IP: orb394740, 5 ug; Detection: orb394740 1:500) with mouse heart tissue lysate 4000 ug.

Various lysates were subjected to SDS PAGE followed by western blot with orb394740 (ATP5A1 antibody) at dilution of 1:1000.

Immunohistochemistry of paraffin-embedded human liver using orb394740 (ATP5A1 antibody) at dilution of 1:50.

Quick Database Links

Gene Symbol

ATP5F1A

UniProt

P25705

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Flow Cytometry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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