BAM7

SKU: orb1305464

Description

BAM 7 is a small molecule that directly and selectively activates the pro-apoptotic protein Bax with an EC50 of 3.3 µM. It serves as a valuable research tool for studying mitochondrial apoptosis pathways in both cellular and biochemical assays, facilitating investigations into cancer biology and therapeutic strategies targeting Bcl-2 family proteins.

Research Area

Cell Biology

Images & Validation

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Key Properties

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CAS Number	331244-89-4
MW	405.47
Purity	98.51% (May vary between batches)
Formula	C₂₁H₁₉N₅O₂S
SMILES	CCOc1ccccc1N\N=C1/C(C)=NN(C1=O)c1nc(cs1)-c1ccccc1
Target	Bcl-2 Family
Solubility	DMSO:4.1 mg/mL (10.11 mM)

Bioactivity

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Target IC50	Bax:3.3 mM(EC50)
In Vivo	In vitro experiments show that BAM7 induces BAX-mediated pore formation, BAX oligomerization, and BAX-dependent cell death. By activating BAX within cells, BAM7 selectively triggers BAX-mediated apoptosis. Its efficacy is limited to cells containing BAX, where it induces the biochemical and morphological hallmarks of BAX-mediated apoptosis. BAM7 binds directly to the N-terminal BH3 domain of BAX, facilitating interaction at the surface and BAX activation via the BIM BH3 helix. Moreover, BAM7 dose-dependently and temporally triggers the conversion of BAX from monomers to oligomers. Read more...
Cell Research	MEFs (2.5 × 103 cells per well) are seeded in 96-well opaque plates for 18-24 h and then incubated with serial dilutions of BAM7, ANA-BAM16 or vehicle (0.15% (v/v) DMSO) in DMEM at 37 °C in a final volume of 100 μL. Cell viability is assayed at 24 h by addition of CellTiter-Glo reagent according to the manufacturer's protocol, and luminescence is measured using a SpectraMax M5 microplate reader. Viability assays are performed in at least triplicate, and the data are normalized to vehicle-treated control wells.(Only for Reference) Read more...

Storage & Handling

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Storage	-20°C
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Inhibitor, inhibit, Bcl-2 Family, Bax, BAM 7, BAM7, BAM-7

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Compound Identifiers

PubChem CID

3101542 ↗

Key Properties

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No computed properties available.

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BAM7 (orb1305464)

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Applications:	ELISA, WB
Reactivity:	A. thaliana
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

BAM7

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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BAM7 (orb1305464)

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