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BAT1 Antibody (C-term)
SKU: orb1928310
Description
Research Area
Molecular Biology
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution range | WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Drosophila, Gallus, Porcine |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 48991 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Alternative Names
−BAT1, UAP56
Similar Products
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BAT1 Antibody (C-term) [orb2995849]
FC, IHC-P, WB
Bovine, Drosophila, Gallus
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
50 μl, 100 μl
Quality Guarantee
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BAT1 Antibody (C-term) western blot analysis in A431, Hela, Jurkat cell line lysates (35 ug/lane).This demonstrates the BAT1 antibody detected the BAT1 protein (arrow).
BAT1 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human kidney reacted with BAT1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
All lanes: Anti-BAT1 Antibody (C-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 49 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Quick Database Links
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UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
BAT1 Antibody (C-term) (orb1928310)
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