Recombinant Bax Antibody

SKU: orb1824648

Description

Recognizes a protein of 21kDa, identified as the Bax protein. This mAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

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Tested Applications	WB
Dilution range	Western blot: 1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the recombinant Bax antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	BAX/8960R
Immunogen	A portion of amino acids 1-100 from human BAX protein was used as the immunogen for the recombinant Bax antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Western blot testing of human A549 cell lysate with recombinant Bax antibody (clone BAX/8960R). Predicted molecular weight ~21 kDa.

SDS-PAGE analysis of purified, BSA-free Bax antibody (clone BAX/8960R) as confirmation of integrity and purity.

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UniProt

Q07812

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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