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Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 5
| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | WB 1:2000, IHC 1:150 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Full length human recombinant protein of human BCL2 (NP_000624) produced in E.coli. |
| Target | BCL2 |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Bcl-2; PPP1R50
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Western blot analysis of extracts (35ug) from 5 cell lines lysates by using anti-BCL2 monoclonal antibody (1:500).
Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-BCL2 monoclonal antibody (1:200).
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-BCL2 mouse monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BCL2 mouse monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL2 (RC204498, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL2.
Quick Database Links
Gene Symbol
BCL2
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
BCL2 Antibody (orb1331720)
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