Bcl2A1 Rabbit Polyclonal Antibody

SKU: orb317564

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Description

Images & Validation

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Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Mouse, Rat
Predicted Reactivity	Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from mouse Bcl2A1 (11-80/172aa)
Target	BCL2A1
Molecular Weight	29 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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ACC 1; ACC 2; B2LA1_HUMAN; Bcl 2 related protein A1; Bcl-2-like protein 5; Bcl-2-related protein A1; BCL2 related protein A1; Bcl2-L-5; BCL2L5; BFL1; GRS; HBPA1; Hematopoietic BCL2 related protein A1; Hemopoietic specific early response protein; Hemopoietic-specific early response protein; Protein BFL 1; Protein BFL-1; Protein GRS.

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Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-Bcl2A1 (orb317564) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 29kD, Observed band size: 29kD.

Tissue/Cell: rat spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Bcl2A1 Polyclonal Antibody, Unconjugated (orb317564) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

BCL2A1

UniProt

Q07440

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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