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BECN1 Antibody (Ascites)
SKU: orb652057
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Description
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−Item 1 of 3
| Tested Applications | IF, IHC-P, WB |
|---|---|
| Dilution range | WB - 1:500-1000, IHC-P - 1:100-500, IF - 1:10-50 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgM, κ |
| Immunogen | Recombinant Protein |
| Molecular Weight | 51896 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
| Disclaimer | For research use only |
Alternative Names
−GT197
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Formalin-fixed and paraffin-embedded human brain with BECN1 Monoclonal Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunofluorescence analysis of BECN1 Monoclonal Antibody with paraffin-embedded human brain tissue.0.05 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits orange fluorescence.
Western blot analysis of anti-BECN1 Monoclonal Antibody in Jurkat cell line lysates (35 μg/lane). BECN1 (arrow) was detected using the Mab ascites (1:100 dilution).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
BECN1 Antibody (Ascites) (orb652057)
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