Beta Amylase Antibody Biotin Conjugated

SKU: orb344948

Description

Beta Amylase antibody (Biotin)

Images & Validation

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Tested Applications	DOT, ELISA, WB
Dilution range	ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
Reactivity	Plant
Application Notes	Anti-Beta Amylase Biotin Conjugated Antibody has been tested by dot blot and western blot. This product is assayed against 1.0 ug of b-Amylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Beta Amylase [Sweet Potato]
Purity	Anti-Beta Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified b-Amylase [Sweet Potato]. Cross reactivity against b-Amylase from other sources may occur but have not been specifically determined.
Conjugation	Biotin

Storage & Handling

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Storage	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL
Dry Ice Shipping	Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
Disclaimer	For research use only

Alternative Names

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rabbit anti-Beta Amylase Antibody biotin Conjugation, biotin conjugated rabbit anti-Beta Amylase Antibody, 1-4 alpha D glucan maltohydrolase antibody, 14 alpha D glucan maltohydrolase antibody, AMYB antibody, BMY1 antibody

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Dot Blot results of Rabbit Anti-Beta Amylase Biotin Conjugated. Antigen: Beta Amylase. Blot loaded at 3 fold dilution: 1. 100 ng, 2. 33.3 ng, 3. 11.1 ng, 4. 3.70 ng, 5. 1.23 ng. Blocking: orb348637 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Beta Amylase Biotin 1 µg/ml for 1hr at RT. Secondary Antibody: Goat anti-Rabbit HRP (p/n orb347654) at 1:70000 for 30 min at RT.

Quick Database Links

UniProt

P10537

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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DOT

Dot Blot

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