Bevacizumab ELISA Kit

SKU: orb2975769

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Human VEGFA has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Bevacizumab in the sample competitively binds to the pre-coated protein with biotin-labeled Bevacizumab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Bevacizumab bound in the initial step. The color development is stopped and the intensity of the color is measured.

Images & Validation

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Key Properties

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Sample Types	Plasma, Serum
Assay Type	Quantitative
Range	312.5 - 20,000 ng/mL
Sensitivity	98.1 ng/mL

Storage & Handling

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Storage	When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20°C, the rest reagents should be store at 4°C.
Disclaimer	For research use only

Alternative Names

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12-IgG1, F(ab)-12 IgG1, Fab-12 IgG1, rhuMAb-VEGF, ABP 215, CAS: 216974-75-3

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