Biotinylated Human B7-H4

SKU: orb2993778

Description

Biotinylated Recombinant Human B7 Homolog 4 is produced by our Mammalian expression system and the target gene encoding Phe29-Ala258 is expressed with a human IgG1 Fc, Avi tag at the C-terminus.

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Origin	Human
Biological Activity	ELISA: Immobilized Anti-Human B7-H4 mAb(Cat#orb2993105) at 2μg/ml (100 μl/well) can bind Biotinylated Human B7-H4-Fc-Avi(Cat#orb2993778). The ED50 of Biotinylated Human B7-H4-Fc-Avi(Cat#orb2993778) is 0.23 ng/ml.. BLI: Loaded Biotinylated Human B7-H4 (C-Fc-Avi) (Cat#orb2993778) on SA Biosensor, can bind Human B7-H4 mAb (Cat#orb2993105) with an affinity constant of 1.24 pM as determined in BLI assay. (Regularly tested)
Tag	C-Fc-Avi
Molecular Weight	Predicted: 54.1 KDa. Observed: 70-95 KDa, reducing conditions
Expression Region	Phe29-Ala258
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Biotin

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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B7S1; B7x; Vtcn1; B7h.5; B7-H4; B7H4T-cell costimulatory molecule B7x; B7S1VCTN1; B7XPRO1291; FLJ22418; Immune costimulatory protein B7-H4; Protein B7S1; T cell costimulatory molecule B7x; V-set domain containing T cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1

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UniProt

Q7Z7D3

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