Biotinylated Human IL-2

SKU: orb2993791

Description

Biotinylated Recombinant Human Interleukin-2 is produced by our Mammalian expression system and the target gene encoding Ala21-Thr153 is expressed with a 6His, Avi tag at the C-terminus.

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Origin	Human
Biological Activity	ELISA: Immobilized Human IL-2RA-His(Cat#orb2994464) at 2μg/ml (100 μl/well)can bind Human IL-2-His-Avi(Cat#orb2993791). The ED50 of Human IL-2-His-Avi(Cat#orb2993791) is 6.45 ng/ml.. BLI: Loaded Human IL-2RA-Fc(Cat#orb2994463) on Protein A Biosensor, can bind Biotinylated Human IL-2-His-Avi(Cat#orb2993791) with an affinity constant of 8.60 nM as determined in BLI assay. Loaded Human IL-2RB-Fc(Cat#orb2994461) on Protein A Biosensor, can bind Biotinylated Human IL-2-His-Avi(Cat#orb2993791) with an affinity constant of 0.24 uM as determined in BLI assay. Loaded Mouse IL-2RA-Fc(Cat#orb2994437) on Pro A Biosensor, can bind Biotinylated Human IL-2-His-Avi(Cat#orb2993791) with an affinity constant of 15.6 nM as determined in BLI assay.. Cell Based Assay: Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 0.2 ng/ml.
Tag	C-6His-Avi
Molecular Weight	Predicted: 18.3 KDa. Observed: 16-18 KDa, reducing conditions
Expression Region	Ala21-Thr153
Purity	SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Biotin

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Disclaimer	For research use only

Alternative Names

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Interleukin-2; IL-2; T-Cell Growth Factor; TCGF; Aldesleukin; IL2

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UniProt

P60568

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No UniProt data available

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