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Recombinant Human LILRB3 Protein (Biotin)

SKU: orb2992836

Description

Biotinylated Recombinant Human Leukocyte immunoglobulin-like receptor subfamily B member 3 is produced by our Mammalian expression system and the target gene encoding Gly24-Glu443 is expressed with a human IgG1 Fc, Avi tag at the C-terminus.

Images & Validation

Key Properties

Expression SystemHuman Cells
Biological OriginHuman
Biological ActivityELISA: Immobilized Anti-LILRB3 mAb(Human IgG1) at 2μg/ml (100 μl/well) can bind Biotinylated Human LILRB3 (C-Fc-Avi)(Cat#orb2992836). The ED50 of Biotinylated Human LILRB3 (C-Fc-Avi)(Cat#orb2992836) is 0.296 ng/ml.(Regularly tested). BLI: Loaded Biotinylated Human LILRB3 (C-Fc-Avi) (Cat#orb2992836) on SA Biosensor, can bind Anti-LILRB3 mAb (Human IgG1) with an affinity constant of 1.11 nM as determined in BLI assay. (Regularly tested)
TagC-Fc-Avi
Molecular WeightPredicted: 74.8 KDa. Observed: 80-120 KDa, reducing conditions
Expression RegionGly24-Glu443
PuritySDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxins< 1 EU/µg as determined by LAL test.

Storage & Handling

StorageLyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/AppearanceLyophilized
Buffer/Preservatives0.2 μm filtered solution of PBS, pH 7.4.
ReconstitutionAlways centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Leukocyte immunoglobulin-like receptor subfamily B member 3; LILRB3; CD85 antigen-like family member A; Monocyte inhibitory receptor HL9; CD85a; ILT5; LIR3

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Recombinant Human LILRB3 Protein (Biotin) (orb2992836)

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