Biotinylated Human Nectin-2

SKU: orb2993711

Description

Biotinylated Recombinant Human Poliovirus Receptor-Related Protein 2 is produced by our Mammalian expression system and the target gene encoding Gln32-Leu360 is expressed with a Avi, 6His tag at the C-terminus.

Images & Validation

−

Key Properties

−

Expression System	Human Cells
Biological Origin	Human
Biological Activity	ELISA: Immobilized Human PVRIG-mFc(Cat#orb2993726) at 10μg/ml (100 μl/well) can bind Biotinylated Human Nectin-2-His-Avi(Cat#orb2993711) The ED50 of Biotinylated Human Nectin-2-His-Avi(Cat#orb2993711) is 0.42 ug/ml. Immobilized Human PVRIG-Fc(Cat#orb2993712) at 10μg/ml (100 μl/well) can bind Biotinylated Human Nectin-2-His-Avi(Cat#orb2993711) The ED50 of Biotinylated Human Nectin-2-His-Avi(Cat#orb2993711) is 0.21 ug/ml.. BLI: Loaded Human PVRIG-Fc(Cat#orb2993712) on His Biosensor, can bind Biotinylated Human Nectin-2(Cat#orb2993711) with an affinity constant of 144.9n M as determined in BLI assay.
Tag	C-6His-Avi
Molecular Weight	Predicted: 38.4 KDa. Observed: 45-60 KDa, reducing conditions
Expression Region	Gln32-Leu360
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	< 1 EU/µg as determined by LAL test.
Conjugation	Biotin

Storage & Handling

−

Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

Poliovirus Receptor-Related Protein 2; Herpes Virus Entry Mediator B; Herpesvirus Entry Mediator B; HveB; Nectin-2; CD112; PVRL2; HVEB; PRR2

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

UniProt

Q92692

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Find More Protocols