Biotinylated Human TGF-beta 1

SKU: orb3002438

Description

Biotinylated Recombinant Human Transforming Growth Factor beta 1 is produced by our Mammalian expression system and the target gene encoding Ala279-Ser390 is expressed with a Avi tag at the N-terminus.

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Activity	BLI: Loaded Biotinylated Human TGF-beta 1(Cat#orb3002438) on SA Biosensor, can bind Recombinant Human TGFBR1(Cat#orb2992807) with an affinity constant of 0.788 uM as determined in BLI assay. (Regularly tested) Loaded Biotinylated Human TGF-beta 1(Cat#orb3002438) on SA Biosensor, can bind Recombinant Human TGFBR2(Cat#orb3002421) with an affinity constant of 0.927nM as determined in BLI assay. (Regularly tested). ELISA: Immobilized Biotinylated Human TGF-beta 1-Avi(Cat#orb3002438) at 2μg/ml (100 μl/well) can bind Human TGFBR2-Fc(Cat#orb3002421).The ED50 of Human TGFBR2-Fc(Cat#orb3002421) is 9.47ng/ml. (Regularly tested). Cell Based Assay: Measured by its ability to inhibit TGF-beta 1 activity on TF‑1 human erythroleukemic cells.The ED50 for this effect is 137.37pg/ml. (Regularly tested)
Tag	N-Avi
Molecular Weight	Predicted: 14.6 KDa. Observed: 12-18 KDa, reducing conditions
Expression Region	Ala279-Ser390
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxins	< 0.01 EU/µg as determined by LAL test.

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized from a 0.2 μm filtered solution of 50mM Glycine-HCl, 150mM NaCl, pH 2.5.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Disclaimer	For research use only

Alternative Names

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Transforming Growth Factor Beta-1; TGF-Beta-1; Latency-Associated Peptide; LAP; TGFB1; TGFB

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UniProt

P01137

UniProt Details

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No UniProt data available

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