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BRCA1 Antibody
SKU: orb1261228
Featured
Description
Research Area
Cancer Biology, Cell Biology
Images & Validation
−Item 1 of 11
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide of human BRCA1 |
| Target | BRCA1 |
| Molecular Weight | Observed: 300kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Breast cancer type 1 susceptibility protein, 632-, RING finger protein 53, BRCA1, RNF53
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Western blot analysis of extracts of various cell lines, using BRCA1 antibody (orb1261228) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human breast cancer using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using c-Jun antibody (orb1261262) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded mouse pancreas using BRCA1 antibody (orb1261228) at dilution of 1:100 (40x lens).
Quick Database Links
Gene Symbol
BRCA1
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
BRCA1 Antibody (orb1261228)
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