BST1 Rabbit Polyclonal Antibody

SKU: orb100702

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Description

Research Area

Cell Biology

Images & Validation

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Item 1 of 2

Tested Applications	FC, WB
Dilution Range	WB=1:500-2000, Flow-Cyt=1μg/Test
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Porcine, Rabbit, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human BST1/CD157 (51-150/318aa)
Target	BST1
Molecular Weight	33 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD157; cADPR2; BST1_HUMAN; BST1; ADP-ribosyl cyclase 2; Bone marrow stromal cell antigen 1 (BST-1); Cyclic ADP-ribose hydrolase 2 (cADPR hydrolase 2); 3.2.2.6;

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Blank control: U937 (blue). Primary Antibody: Rabbit Anti-BST1 antibody (orb100702), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb100702, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Sample: Raw264.7 (Mouse) Cell Lysate at 30 ug, Primary: Anti-BST1 (orb100702) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33 kD, Observed band size: 33 kD.

Quick Database Links

Gene Symbol

BST1

UniProt

Q10588

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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