Calbindin 1 antibody

SKU: orb2276325

Description

Calbindin 1 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

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Item 1 of 3

Tested Applications	IHC
Dilution range	1:50-1:100
Reactivity	Human
Application Notes	Positive Control: Kidney: A subset of the cells of distal tubuli should show an intense cytoplasmic calbindin 1 immunostaining. Negative Control: Colon: Calbindin 1 immunostaining should be completely absent in all cells. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions. Read more...

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG2c, kappa
Clone No.	MSVA-471M
Immunogen	Recombinant fragment (around aa 7-96) of human CALB1 protein (exact sequence is proprietary)
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

Alternative Names

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avian-type; CAB27; CALB 1; CALB; CALB1; CALB1_HUMAN; Calbindin 1 28kDa; Calbindin; Calbindin D28; D 28K; D-28K; D28K; OTTHUMP00000166027; OTTHUMP00000225441; RTVL H protein; Vitamin D dependent calcium binding protein; Vitamin D dependent calcium binding protein avian type; Vitamin D-dependent calcium-binding protein

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Cerebellum molecular layer Purkinje cell layer granule cell layer white matter A strong Calbindin 1 staining of Purkinje cells and of associated axonal fibres is seen in the cerebrum.

Colon descendes mucosa Calbindin 1 immunostaining is absent in all cells of the colon mucosa.

Kidney cortex A strong cytoplasmic Calbindin 1 immunostaining occurs in a fraction of distal tubuli of the kidney.

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UniProt

P05937

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No UniProt data available

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Protocol Information

IHC

Immunohistochemistry

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