calbindin D28 Antibody

SKU: orb12289

Description

Goat Polyclonal antibody to Calbindin

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:128000, WB: 0.03-0.1 μg/ml, IHC-P: 5 μg/ml
Reactivity	Human, Rat
Predicted Reactivity	Bovine, Canine, Porcine
Application Notes	WB: Approx. 26kDa band observed in Human and Rat Kidney lysates and in Rat Brain lysates (calculated MW of 30.0kDa according to Human NP_004920.1and Rat NP_114190.1). The observed molecular weight is routinely observed by other sources. Recommended concentrat. IHC: Paraffin embedded Human Kidney. Recommended concentration: 5-7µg/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	calbindin D28
Molecular Weight	30
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Disclaimer	For research use only

Alternative Names

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CALB1; calbindin 1, 28kDa; CALB; D-28K; OTTHUMP00000166027; OTTHUMP00000225441; RTVL-H protein; calbindin; calbindin 1, (28kD); calbindin D28; vitamin D-dependent calcium-binding protein, avian-type

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Western blot analysis of staining of Human Cerebellum lysate using CALB1 antibody.

Immunohistochemical analysis of staining of Human Cerebellum lysate using Calbindin antibody

Western blot analysis of staining of Mouse (A) and Rat (B) Brain lysates using CALB1 antibody.

Immunohistochemical staining of Human Kidney using Calbindin antibody

Quick Database Links

Gene Symbol

calbindin D28

NCBI Gene ID

793

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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