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Caldesmon Antibody / CALD1 / CAD (HMW)
SKU: orb1151813
Description
Images & Validation
−Item 1 of 3
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | Mouse IgG1, kappa |
| Clone No. | rCALD1/820 |
| Immunogen | Recombinant full-length human CALD1 protein was used as the immunogen for the recombinant Caldesmon antibody. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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Caldesmon Antibody / CALD1 / CAD (HMW) [orb1151444]
IHC-P
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Mouse
Recombinant
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20 μg, 100 μg
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Western blot testing of human ovary tissue lysate using recombinant Caldesmon antibody (clone rCALD1/820). Predicted molecular weight ~93 kDa, can be observed at 70-80 kDa (non muscle tissue) and 120-150 kDa (smooth muscle).
IHC staining of FFPE human leiomyosarcoma tissue with recombinant Caldesmon antibody (clone rCALD1/820). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Caldesmon antibody (clone rCALD1/820) as confirmation of integrity and purity.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
Caldesmon Antibody / CALD1 / CAD (HMW) (orb1151813)
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