Calpain 2 Rabbit Polyclonal Antibody

SKU: orb10220

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Description

Calpain 2 Rabbit Polyclonal Antibody

Research Area

Cell Biology

Images & Validation

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Item 1 of 3

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Rat, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from mouse Calpain 2 (301-400/700aa)
Target	CAPN2
Molecular Weight	78 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CANP2; CANPL2; CANPml; mCANP; CAN2_HUMAN; CAPN2; Calcium-activated neutral proteinase 2 (CANP 2); Calpain M-type; Calpain large polypeptide L2; Calpain-2 large subunit; Millimolar-calpain (M-calpain); 3.4.22.53;

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Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Calpain 2) Polyclonal Antibody, Unconjugated (orb10220) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Brain (Mouse) Lysate at 40 ug, Primary: Anti-Calpain 2 (orb10220) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 78 kD, Observed band size: 90 kD.

Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-Calpain 2 (orb10220) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 78 kD, Observed band size: 78 kD.

Quick Database Links

Gene Symbol

CAPN2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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