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CASP1/Caspase 1 Antibody

SKU: orb1535298

Description

CASP1/Caspase 1 Antibody

Research Area

Cell Biology

Images & Validation

Tested ApplicationsICC, IHC, IHC-P, WB
Dilution RangeICC, IHC, IHC-P (20 µg/ml), WB (0.5 - 2 µg/ml)
ReactivityHuman, Mouse
Application Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 20 ug/ml.

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG1,k
Clone No.14F468
ImmunogenTwo synthetic peptides from the human caspase-1 protein (aa 371-390 and aa 31-45)
TargetCASP1 / Caspase 1
PurificationProtein G purified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS, 0.02% Sodium Azide
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CASP1, Caspase-1, CASP-1, CASP1 nirs variant 1, Caspase 1, ICE, ICE-like protease, IL-1 beta-converting enzyme, IL-1BC, IL1BC, IL1BCE, p45, IL1B-convertase, Interleukin-1 beta convertase

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ICC
Immunocytochemistry
View Protocol

CASP1/Caspase 1 Antibody (orb1535298)

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Available Sizes

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50 μg
¥ 8,320.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 2-3 weeks
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