Cattle Heat Shock Protein 90kDa Alpha B1 (HSP90aB1) ELISA Kit

SKU: orb781856

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 90kDa Alpha B1(HSP90aB1).

Research Area

Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle HSP90aB1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle HSP90aB1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle HSP90aB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle HSP90aB1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Bovine
Sample Types	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.057 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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HSP90-BETA ELISA Kit, HSP90B ELISA Kit, HSPC2 ELISA Kit, HSPCB ELISA Kit, HSP84 ELISA Kit, Heat Shock 90kD Protein 1,Beta ELISA Kit, Heat shock 84 kDa ELISA Kit

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Cattle Heat Shock Protein 90kDa Alpha B1 (HSP90aB1) ELISA Kit

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UniProt

Q76LV1

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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