Cattle Luteinizing Hormone (LH) ELISA Kit

SKU: orb781157

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Luteinizing Hormone(LH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Luteinizing Hormone(LH).

Research Area

Metabolism Research, Stem Cell & Developmental Biology

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 100 mIU/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle LH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle LH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle LH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle LH in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Bovine
Sample Types	Serum, plasma and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	468.75-30000 pg/mL
Sensitivity	161.5 pg/mL
Concentration	100 mIU/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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ICSH ELISA Kit, Lutropin ELISA Kit, Interstitial Cell Stimulating Hormone ELISA Kit

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Cattle Luteinizing Hormone (LH) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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