Cattle S100 Calcium Binding Protein A9 (S100A9) ELISA Kit

SKU: orb781813

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A9(S100A9).

Research Area

Cancer Biology, Infectious Disease & Virology, Musculoskeletal & Connective Tissue Research

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle S100A9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle S100A9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle S100A9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle S100A9 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Bovine
Sample Types	Serum, plasma and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.1 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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MRP14 ELISA Kit, MIF ELISA Kit, 60B8AG ELISA Kit, CFAG ELISA Kit, NIF ELISA Kit, CAGB ELISA Kit, CGLB ELISA Kit, L1AG ELISA Kit, P14 ELISA Kit, Calgranulin B ELISA Kit, Migration inhibitory factor-related protein 14 ELISA Kit, Calprotectin L1H subunit ELISA Kit, Leukocyte L1 complex heavy chain ELISA Kit

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Cattle S100 Calcium Binding Protein A9 (S100A9) ELISA Kit

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UniProt

P28783

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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