Cattle Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit

SKU: orb779316

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Transforming Growth Factor Beta 1(TGFb1) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 1(TGFb1).

Research Area

Cancer Biology, Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle TGFb1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle TGFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle TGFb1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Bovine
Sample Types	serum, platelet-poor plasma, cell culture supernates and other biological fluids
Assay Type	Competitive
Assay Time	2h
Range	15.63-1000 pg/mL
Sensitivity	4.8 pg/mL
Concentration	1000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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TGF Beta 1,TGF-B1 ELISA Kit, CED ELISA Kit, DPD1 ELISA Kit, LAP ELISA Kit, Camurati-Engelmann Disease ELISA Kit, Latency-associated peptide ELISA Kit

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Cattle Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit

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UniProt

P18341

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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