Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human CBX4 (401-500/560aa)
Target	CBX4
Molecular Weight	61 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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CBX 4; CBX4_HUMAN; E3 SUMO-protein ligase CBX4; EC:2.3.2.-; Chromobox protein homolog 4; Polycomb 2 homolog(Pc2; hPc2); Pc2; hPc2;

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Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (CBX4) polyclonal Antibody, Unconjugated (orb182925) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: Mouse Liver tissue lysates, Lane 2: Rat Liver tissue lysates, Lane 3: Human K562 cell lysates, Lane 4: Human HL-60 cell lysates, Lane 5: Human HeLa cell lysates, Lane 6: Human HepG2 cell lysates, Lane 7: Human HUVEC cell lysates, Lane 8: Human A549 cell lysates, Primary: Anti-CBX4 (orb182925) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 61 kDa, Observed band size: 61 kDa.

Quick Database Links

Gene Symbol

CBX4

UniProt

O00257

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ICC

Immunocytochemistry

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