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CD11c Antibody

SKU: orb749612

Description

Recognizes a protein of 145 kDa, identified as CD11c. CD11c (ITGAX), a member of the leukointegrin family, shares the same beta subunit with other members of the leukocyte adhesion molecule family, which includes CD11a (LFA-1), CD11b (MAC-1) and CD11d (ITGAD), but has a unique alpha chain. CD11c has been shown to play a role in phagocytosis, cell migration, and cytokine production by monocytes/macrophages as well as induction of T-cell proliferation by Langerhans cells. CD11c is expressed prominently on the plasma membranes of monocytes, tissue macrophages, NK cells, and most dendritic cells (DCs). A lower level of expression is also observed on neutrophils as a result of its high level of expression on most DCs. An antibody to CD11c may aid in identification of lesions with histiocytic origin. It may also been used as a marker for hairy cell leukemia in paraffin-embedded tissues.

Research Area

Epigenetics

Images & Validation

Tested ApplicationsIHC-P
Dilution rangeImmunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
ReactivityHuman
Application Notes
Optimal dilution of the CD11c antibody should be determined by the researcher.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG2b, kappa
Clone No.ITGAX/1242
ImmunogenA partial recombinant protein corresponding to amino acids 637-827 from the human protein was used as the immunogen for the CD11c antibody.
PurificationProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
DisclaimerFor research use only

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CD11c Antibody

IHC testing of human tonsil with CD11c antibody (clone ITGAX/1242). Note specific staining of dendritic cells. Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.

CD11c Antibody

SDS-PAGE Analysis of Purified, BSA-Free CD11c Antibody (clone ITGAX/1242). Confirmation of Integrity and Purity of the Antibody.

CD11c Antibody

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using CD11c antibody (clone ITGAX/1242). These results demonstrate the foremost specificity of the ITGAX/1242 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

UniProt Details

No UniProt data available

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol

CD11c Antibody (orb749612)

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Available Sizes

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20 μg
¥ 4,810.00
100 μg
¥ 9,750.00
DispatchUsually dispatched within 5-10 working days
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