CD14 Antibody (Macrophage Marker)

SKU: orb534064

Description

Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. [UniProt]

Research Area

Epigenetics, Immunology

Images & Validation

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Tested Applications	IHC-P, WB
Dilution range	IHC (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Reactivity	Human
Application Notes	The stated application concentrations are suggested starting points. Titration of the CD14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	CDLA14-2
Immunogen	A portion of amino acids 25-148 was used as immunogen for this CD14 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC testing of FFPE human lymph node with CD14 antibody (clone CDLA14-2). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human A549 cell lysate with CD14 antibody (clone LPSR/2386). Expected molecular weight: 40-55 kDa depending on glycosylation level.

Quick Database Links

UniProt

P08571

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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