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CD146 antibody
SKU: orb10290
Featured
Description
Images & Validation
−Item 1 of 8
| Tested Applications | ICC, IF, IHC-P, WB |
|---|---|
| Dilution Range | IF/ICC: 1:100-600, IHC-P: 1:100-600, WB: 1:200-1000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human CD146. Please contact us for the exact immunogen sequence. The peptide is available as orb12884. |
| Target | CD146 |
| Molecular Weight | 72 kDa |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti-A32 antigen antibody, anti-CD 146 antibody, anti-CD146 antibody, anti-CD146 antigen antibody, anti-Cell surface glycoprotein MUC18 antibody, anti-Cell surface glycoprotein P1H12 antibody, anti-Gicerin antibody, anti-Mcam antibody, anti-Melanoma adhesion molecule antibody, anti-Melanoma associated antigen A32 antibody, anti-Melanoma associated antigen MUC18 antibody, anti-MelCAM antibody, anti-MUC 18 antibody, anti-MUC18 antibody, anti-S endo 1 antibody, anti-S endo 1 endothelial associated antigen antibody
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Immunohistochemical staining of human breast cancer tissue using CD146 antibody (dilution of primary antibody - 2.5 ug/ml)
Immunohistochemical staining of mouse kidney tissue using anti-CD146 (dilution of primary antibody - 2.5 ug/ml)
Western blot analysis of mouse brain (lane 1), mouse colon (lane 2), mouse kidney (lane 3), rat spleen (lane 4), human breast cancer (lane 5), U251 cells (lane 6),293T cells (lane 7) using CD146 antibody (1 ug/ml)
Immunohistochemical staining of rat brain tissue using CD146 antibody (dilution of primary antibody - 2.5 ug/ml)
IF analysis of mouse kidney tissue using CD146 antibody (dilution of primary antibody at 2.5 ug/ml)
WB analysis of mouse brain (lane 1), mouse colon (lane 2), mouse kidney (lane 3), rat spleen (lane 4), human breast cancer (lane 5), U251 cells (lane 6),293T cells (lane 7) using CD146 antibody (1 ug/ml)
IHC-P staining of mouse kidney tissue using anti-CD146 (dilution at 2.5 ug/ml)
Immunofluorescence image of mouse kidney tissue using anti-CD146 (dilution at 2.5 ug/ml)
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ICC
Immunocytochemistry
CD146 antibody (orb10290)
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