CD19 Recombinant Rabbit Monoclonal Antibody

SKU: orb2563456

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Description

Research Area

CD Markers, Cell Biology, Human Lineage-negative, Stem Cell, Stem Cell Research

Images & Validation

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Item 1 of 2

Tested Applications	FC, ICC, IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:50-200, IF=1:50-200, IHC-F=1:50-200, Flow-Cyt=1:50-100, ICC/IF=1:50-200
Reactivity	Human

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	7A6
Immunogen	A synthesized peptide derived from human CD19 (300-556aa)
Target	CD19
Molecular Weight	59 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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CD19_HUMAN; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD_antigen: CD19; B-lymphocyte surface antigen B4; Leu 12; Leu12;

Quality Guarantee

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CD19 Recombinant Rabbit Monoclonal Antibody

25 ug total protein per lane of various lysates probed with CD19 monoclonal antibody, unconjugated (orb2563456) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Human Tonsil, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD19 Monoclonal Antibody, Unconjugated (orb2563456) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.