CD195 Antibody

SKU: orb2988635

Description

The CD195 Antibody is suitable for FC, IF. It is a Monoclonal, Unconjugated antibody which raised against CHO cells transfected with human CCR5 .Purification: The antibody was purified by affinity chromatography.

Images & Validation

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Tested Applications	FC, IF
Reactivity	Human
Application Notes	1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured). Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Rat
Clonality	Monoclonal
Clone No.	HEK/1/85a
Immunogen	CHO cells transfected with human CCR5
Purification	The antibody was purified by affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Rat IgG2b kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide.
Disclaimer	For research use only

Alternative Names

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CMKBR5; C-C chemokine receptor type 5; C-C CKR-5; CC-CKR-5; CCR-5; CCR5; CHEMR13; HIV-1 fusion coreceptor; CD195

Quality Guarantee

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Quick Database Links

UniProt

P51681

NCBI Gene ID

1234

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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Immunofluorescence

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CD195 Antibody (orb2988635)

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Applications:	FACS, IF, IHC-P
Reactivity:	Human
Species/Host:	Mouse
Clonality:	Monoclonal
Conjugation:	Unconjugated

Applications:	FACS, IF, IHC-P
Reactivity:	Human
Species/Host:	Mouse
Clonality:	Monoclonal
Conjugation:	Unconjugated

Applications:	FC
Reactivity:	Human
Clonality:	Monoclonal
Conjugation:	PE

Applications:	ELISA, WB
Reactivity:	Human, Mouse, Rat
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, IF, IHC, WB
Reactivity:	Human, Mouse, Rat
Clonality:	Polyclonal
Conjugation:	Unconjugated

CD195 Antibody

Description

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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Quality Guarantee

Quick Database Links

UniProt Details

NCBI Gene Details

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Protocol Information

CD195 Antibody (orb2988635)

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