CD23 (Fc Epsilon RII) Antibody

SKU: orb1410377

Description

Mouse monoclonal antibody to CD23 (Fc Epsilon RII)

Images & Validation

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Item 1 of 4

Tested Applications	IHC
Reactivity	Human

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG2b
Clone No.	FCER2/6887
Immunogen	Recombinant fragment (around aa 48-321) of human FCER2/CD23 protein (exact sequence is proprietary)
Target	FCER2
Source/Expression System	HEK293 cells
Molecular Weight	37kDa (soluble form); 45kDa (membrane-bound form)
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Disclaimer	For research use only

Alternative Names

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BLAST-2; C-type lectin domain family 4, member J; Fc fragment of IgE low affinity II receptor; Fc-epsilon-RII; FCER2; FCER2A; FceRII; IgE-binding factor (IGEBF); Immunoglobulin E receptor, low affinity II; Lymphocyte IgE receptor

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Analysis of Protein Array containing more than 19000 full-length human proteins using CD23-Monospecific Mouse Monoclonal Antibody (FCER2/6887). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

IHC analysis of formalin-fixed, paraffin-embedded human lymph node. Membrane staining using FCER2/6887 at 2 ug/ml in PBS for 30 min RT. HIER: Tris/EDTA, pH9.0, 45 min. 2°C: HRP-polymer, 30 min. DAB, 5 min.

IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Membrane staining using FCER2/6887 at 2 ug/ml in PBS for 30 min RT. Inset: PBS instead of primary antibody; secondary only negative control.

SDS-PAGE Analysis of Purified CD23-Monospecific Mouse Monoclonal Antibody (FCER2/6887). Confirmation of Purity and Integrity of Antibody.

Quick Database Links

Gene Symbol

FCER2

UniProt

P06734

UniProt Details

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No UniProt data available

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Protocol Information

IHC

Immunohistochemistry

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