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CD24 Antibody (N-Term)
Description
Research Area
Images & Validation
−| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:500, IHC-P - 1:100-500, FC - 1:10-50 |
| Reactivity | Human |
| Predicted Reactivity | Mouse |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 8083 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Western blot analysis of CD24 Antibody (N-Term) in MCF-7 cell line lysates (35 ug/lane). CD24 (arrow) was detected using the purified Pab.

CD24 Antibody (N-Term) western blot analysis in Raji, Ramos cell line and rat cerebellum tissue lysates (35 ug/lane). This demonstrates the CD24 antibody detected the CD24 protein (arrow).

CD24 Antibody (N-Term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with CD24 Antibody (N-Term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Protocol Information
CD24 Antibody (N-Term) (orb2649267)
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