CD31 Antibody-PerCP labled

SKU: orb2989858

Description

The CD31 Antibody-PerCP labled is suitable for FC, IF. It is a Monoclonal, PerCP conjugated antibody which raised against Native purified human CD31.. Purification: The antibody was purified by affinity chromatography.

Images & Validation

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Tested Applications	FC, IF
Dilution range	10 μl / assay
Reactivity	Human
Application Notes	1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature. 7. Repeat step 5. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured). Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Clone No.	MEM-05
Immunogen	Native purified human CD31.
Purification	The antibody was purified by affinity chromatography.
Conjugation	PerCP

Storage & Handling

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Storage	Shipped and store at 4°C for one year. Do not freeze.
Form/Appearance	Mouse IgG1. Liquid in PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
Disclaimer	For research use only

Alternative Names

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Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31

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Quick Database Links

UniProt

P16284

NCBI Gene ID

5175

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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Immunofluorescence

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