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CD3E Antibody
SKU: orb1333884
Description
Images & Validation
−Item 1 of 9
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution range | WB 1:200~4000, IHC 1:150, IF 1:100 |
| Reactivity | Canine, Human, Monkey |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Immunogen | Full length human recombinant protein of human CD3E(NP_000724) produced in HEK293T cell. |
| Target | CD3E |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
| Disclaimer | For research use only |
Alternative Names
−IMD18; T3E; TCRE
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD3E (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD3E. Positive ly
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD3E monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-CD3E monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min) (1:2000) (1:4000)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min) (1:2000) (1:4000)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min) (1:2000) (1:4000)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min) (1:2000) (1:4000)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
Anti-CD3E mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD3E.
Quick Database Links
Gene Symbol
CD3E
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
CD3E Antibody (orb1333884)
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