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CD45RO Antibody
SKU: orb749384
Description
Images & Validation
−Item 1 of 5
| Tested Applications | FACS, IF, IHC-P |
|---|---|
| Dilution range | Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a, kappa |
| Clone No. | UCHL-1 |
| Immunogen | Cultured human T-cells from an IL-2-dependent T-cell line (CA1) were used as the immunogen for the CD45RO antibody. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 1X PBS, pH 7.4 |
| Disclaimer | For research use only |
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IHC testing of human tonsil stained with CD45RO antibody (UCHL-1).
FACS staining of human lymphocytes using CD45RO antibody (UCHL-1, red) and isotype control (green).
SDS-PAGE Analysis of Purified, BSA-Free CD45RO Antibody (clone UCHL-1). Confirmation of Integrity and Purity of the Antibody.
Immunofluorescent staining of FFPE human tonsil tissue with CD45RO antibody (clone UCHL-1).
Flow cytometry staining of PFA-fixed human Jukat cells with CD45RO antibody; Red = isotype control, Blue = CD45RO antibody.
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Protocol Information
IHC-P
Immunohistochemistry Paraffin
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
IF
Immunofluorescence
CD45RO Antibody (orb749384)
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