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CD47 Antibody / IAP / Integrin Associated Protein
SKU: orb1151572
Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IHC-P |
|---|---|
| Dilution range | ELISA (order BSA-free format for coating),Immunohistochemistry (FFPE): 1-2ug/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | Rabbit IgG, kappa |
| Clone No. | CD47/6362R |
| Immunogen | A portion of amino acids 18-135 from the human protein was used as the immunogen for the recombinant CD47 antibody. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Disclaimer | For research use only |
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IHC staining of FFPE human prostate carcinoma tissue with recombinant CD47 antibody (clone CD47/6362R). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate carcinoma tissue with recombinant CD47 antibody (clone CD47/6362R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CD47 antibody (clone CD47/6362R) as confirmation of integrity and purity.
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Protocol Information
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CD47 Antibody / IAP / Integrin Associated Protein (orb1151572)
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