CD80 Mouse Monoclonal Antibody (FITC)

SKU: orb2989871

Description

The CD80 Antibody-FITC labled is suitable for FC, IF. It is a Monoclonal, FITC conjugated antibody which raised against Native purified human CD80.. Purification: The antibody was purified by affinity chromatography.

Images & Validation

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Tested Applications	FC, IF
Reactivity	Human
Application Notes	1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured). Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1
Clone No.	2D10.4
Immunogen	Native purified human CD80.
Target	CD80
Purification	The antibody was purified by affinity chromatography.
Conjugation	FITC

Storage & Handling

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Storage	Shipped and store at 4°C for one year. Do not freeze.
Form/Appearance	Liquid
Buffer/Preservatives	PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD28LG; CD28LG1; LAB7; T-lymphocyte activation antigen CD80; Activation B7-1 antigen; BB1; CTLA-4 counter-receptor B7.1; B7; CD antigen CD80

Quality Guarantee

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Quick Database Links

Gene Symbol

CD80

UniProt

P33681

NCBI Gene ID

941

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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Immunofluorescence

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