Cdc2 (phospho Tyr15) rabbit pAb

SKU: orb764156

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Description

Rabbit polyclonal antibody to Cdc2 (phospho-Tyr15)

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, WB
Dilution range	Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Reactivity	Human, Monkey, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human CDC2 around the phosphorylation site of Tyr15. AA range:5-54
Molecular Weight	34
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-CDK1 antibody, anti-CDC2 antibody, anti-CDC28A antibody, anti-CDKN1 antibody, anti-P34CDC2 antibody, anti-Cyclin-dependent kinase 1 antibody, anti-CDK1 antibody, anti-Cell division control protein 2 homolog antibody, anti-Cell division protein kinase 1 antibody, anti-p34 protein kinase antibody

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Western blot analysis of lysates from Mouse-brain, Rat-brain, Hela cells treated with nocodazole 1ug/ml 18h, using CDC2 p-Thr15 Antibody. Primary Antibody was diluted at 1:1000 4°C over night, secondary antibody was diluted at 1:10000, 37° 1hour.

Western blot analysis of Cdc2 0.1ug/ml 18hour, 4°C over night, secondary antibody was diluted at 1:10000, 37° 1hour.

Western Blot analysis of various cells using Phospho-Cdc2 (Y15) Polyclonal Antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CDC2 (Phospho-Tyr15) Antibody.

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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