Cdk1/2/3 (phospho Thr14) rabbit pAb

SKU: orb764160

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Description

Rabbit polyclonal antibody to Cdk1/2/3 (phospho-Thr14)

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, WB
Dilution range	Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Reactivity	Human, Monkey, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human CDK1/CDC2 around the phosphorylation site of Thr14. AA range:1-50
Molecular Weight	34
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-CDK1 antibody, anti-CDC2 antibody, anti-CDC28A antibody, anti-CDKN1 antibody, anti-P34CDC2 antibody, anti-Cyclin-dependent kinase 1 antibody, anti-CDK1 antibody, anti-Cell division control protein 2 homolog antibody, anti-Cell division protein kinase 1 antibody, anti-p34 protein kinase antibody, anti-CDK2 antibody, anti-CDKN2 antibody, anti-Cyclin-dependent kinase 2 antibody

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Western Blot analysis of various cells using Phospho-Cdk1/2/3 (T14) Polyclonal Antibody diluted at 1:2000.

Western Blot analysis of COS7 cells using Phospho-Cdk1/2/3 (T14) Polyclonal Antibody diluted at 1:2000.

Immunofluorescence analysis of COS7 cells, using CDK1/CDC2 (Phospho-Thr14) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HepG2 cells treated with Forskolin 40nM 30', using CDK1/CDC2 (Phospho-Thr14) Antibody. The lane on the right is blocked with the phospho peptide.

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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