You have no items in your shopping cart.
Cdk6 Recombinant Rabbit Monoclonal Antibody
SKU: orb559223
Description
Images & Validation
−Item 1 of 3
| Tested Applications | FC, ICC, WB |
|---|---|
| Dilution range | WB=1:500-2000, ICC/IF=1:50-200, Flow-Cyt=1:50-200 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | 4F7 |
| Immunogen | Recombinant human Cdk6 protein, full length |
| Target | CDK6 |
| Molecular Weight | 37 kDa |
| Purification | Affinity purified by Protein A |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−CDK 6; Cell division protein kinase 6; Crk 2; Crk2; Cyclin dependent kinase 6; Cyclin-dependent kinase 6; MGC59692; p40; PLSTIRE; Serine/threonine-protein kinase PLSTIRE; STQTL11; CDK6_HUMAN; PLSTIRE; Serine/threonine protein kinase PLSTIRE.
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Blocking buffer: 5% NFDM/TBST, Primary Ab, dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: Jurkat, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 37 kDa, Observed MW: 37 kDa.
Cell line: HeLa, Fixation: 4% Paraformaldehyde, Permeabilization: 90% Methanol, Primary Ab, dilution: 1:50, Secondary Ab: Goat Anti-Rabbit IgG, Unlabelled control: The cell without incubation, with primary antibody and secondary antibody, (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb559223.
Cell line: HeLa, Fixative: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary Ab, dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb559223.
Quick Database Links
Gene Symbol
CDK6
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ICC
Immunocytochemistry
Cdk6 Recombinant Rabbit Monoclonal Antibody (orb559223)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 5-10 working days