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Description
Research Area
Infectious Disease & Virology
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IF, IHC-P, WB |
|---|---|
| Dilution Range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This CHEK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-141 amino acids from the N-terminal region of human CHEK2. Antigen Region: 111-141 aa. |
| Target | CHEK2 (HGNC:16627) |
| Molecular Weight | 60915 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CHEK2; CDS1; CHK2; RAD53; Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog; Checkpoint kinase 2
Similar Products
−- Item 1 of 5
CHEK2 Antibody (N-term) [orb1930721]
FC, IF, IHC-P, WB
Human
Rabbit
Polyclonal
Unconjugated
100 μl, 50 μl
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CHEK2 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysates from K562, NCI-H460, Jurkat cell line (from left to right), using CHEK2 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.
Confocal immunofluorescent analysis of CHEK2 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
CHEK2 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CHEK2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Quick Database Links
Gene Symbol
CHEK2 (HGNC:16627)
UniProt
RefSeq (Protein):NP_009125.1, NP_665861.1, NP_001005735.1, NP_001244316.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_009125.1, NP_665861.1, NP_001005735.1, NP_001244316.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IF
Immunofluorescence
CHEK2 Antibody (N-term) (orb1788224)
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